Upregulated circular RNA circ_0007534 indicates an unfavorable prognosis in pancreatic ductal adenocarcinoma and regulates cell proliferation,apoptosis, and invasion by sponging miR-625 and miR-892b |
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Authors: | Liguo Hao Wei Rong Lianjie Bai Hongsheng Cui Shuli Zhang Yuanchun Li Datong Chen Xin Meng |
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Institution: | 1. Department of Imaging Equipment and Technology, Medical Technology Academy, Qiqihar Medical University, Heilongjiang, China;2. Micromorphology Research Center, Medical Pathology Academy, Qiqihar Medical University, Heilongjiang, China;3. Department of Ultrasound, The Second Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China;4. Department of Magnetic Resonance, The Third Affiliated Hospital of Qiqihar Medical University, Heilongjiang, China |
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Abstract: | Circular RNAs (circRNAs) have been regarded as critical regulators of human diseases and biological markers in some types of malignancies, including pancreatic ductal adenocarcinoma (PDAC). Recently, circ_0007534 has been identified as a novel cancer-related circRNA. Nevertheless, its clinical relevance, functional roles, and mechanism have not been studied in PDAC. In the current study, real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of circ_0007534 in 60-paired PDAC tissue samples and different cell lines. Loss-of-function and gain-of-function assays were performed to detect cell proliferation, apoptosis, and metastatic properties affected by circ_0007534. An animal study was also carried out. The luciferase reporter assay was performed to uncover the underlying mechanism of circ_0007534. As a result, circ_0007534 was overexpressed not only in PDAC tissues but also in a panel of PDAC cell lines, and this overexpression is closely associated with advanced tumor stage and positive lymph node invasion. In addition, circ_0007534 may be regarded as an independent prognostic factor for patients with PDAC. For the part of functional assays, circ_0007534 significantly increased cell proliferation, migratory, and invasive potential of PDAC cells. Circ_0007534 could inhibit cell apoptosis partly via a Bcl-2/caspase-3 pathway. The xenograft study further confirmed the cell growth promoting the role of circ_0007534. Mechanistically, miR-625 and miR-892b were sponged by circ_0007534. The oncogenic functions of circ_0007534 is partly dependent on its regulation of miR-625 and miR-892b. In conclusion, our study illuminates a novel circRNA that confers an oncogenic function in PDAC. |
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Keywords: | circ_0007534 circular RNA miR-625 miR-892b pancreatic ductal adenocarcinoma |
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