Overexpression of Hsc70 promotes proliferation,migration, and invasion of human glioma cells |
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| Authors: | Guan Sun Ying Cao Yitian Xu De Huai Ping chen Jun Guo Min Li Yuyu Dai |
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| Affiliation: | 1. Department of Neurosurgery, Yancheng City No. 1 People’s Hospital, The Fourth Affiliated Hospital of Nantong University, Yancheng, P. R. China;2. Department of Ear-Nose-Throat, The Second People’s Hospital of Huai’an, Huai’an Affiliated Hospital of Xuzhou Medical University, Huai’an, P. R. China;3. The First School of Clinical Medicine, Nanjing Medical University, Nanjing, Jiangsu, P. R. China;4. Department of Oncology, Yancheng City No. 1 People’s Hospital, The Fourth Affiliated Hospital of Nantong University, Yancheng, P. R. China;5. Department of Neurosurgery, Jiangning Hospital Affiliated with Nanjing Medical University, Nanjing, P. R. China;6. Department of Neurosurgery, Yancheng Third People’s Hospital, Yancheng, P. R. China |
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| Abstract: | Migration and invasion are often recognized as the main reasons for the high recurrence and death rates of glioma and limit the efficacy of surgery and other antitumor therapies. In this study, we found over activation of heat shock cognate protein 70 (Hsc70) in human glioma specimens, which was closely related to glioma grade. We investigated whether Hsc70 induced the migration and invasion of glioma cells. Wound healing and transwell migration assay were used to determine the migration and invasion ability of human glioma U251 and U87 cells, in which the expression of Hsc70 was knocked down by small interfering RNA. Western blot analysis was performed to determine the expression of FAK-Src signaling in malignant glioma cells. The results showed that Hsc70 deficiency significantly retarded migration and invasion and reduced the phosphorylation of FAK, Src, and Pyk2 in U251 and U87 cells. Overall, our results indicate that the migration and invasion capacity of human brain glioma cells is at least partly induced by Hsc70-dependent activation of FAK-Src signaling. |
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| Keywords: | FAK-Src signaling glioma Hsc70 invasion migration |
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