T helper cells subtypes and their cytokine gene expression affected by carvacrol in sensitized mice administered during sensitization period |
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Authors: | Majid Kianmehr Abdolrahim Rezaee Mahmoud Mahmoudi Vahideh Ghorani Mohammad Hossein Boskabady |
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Affiliation: | 1. Esfarayen Faculty of Medical Sciences, Esfarayen, Iran;2. Division of Inflammation and Inflammatory Diseases, Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;3. Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran Department of Immunology and Allergy, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;4. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran;5. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran |
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Abstract: | Th1, Th2, Th17, and Treg cells and their cytokine gene expressions in splenocytes of control mice, ovalbumin sensitized (S), and S treated with dexamethasone and carvacrol during a sensitization period were examined. Th2 and Th17 population as well as the gene expression of IL-4, IL-17, and TGF-β were increased, but Th1, Th1/Th2 ratio, the gene expression of IFN-γ and FOXP3 as well as the IFN-γ/IL-4 ratio were decreased in S compared with control group ( P < 0.001 for all cases). Carvacrol treatment caused significant reduction of Th2 and Th17 population as well as gene expression of IL-4, IL-17, and TGF-β but increase in Treg cells, Th1/Th2 ratio, gene expressions of FOXP3, IFN-γ, and IFN-γ/IL-4 ratio ( P < 0.05 to P < 0.001). The population of Th1, Th2, Th17 cells as well as the gene expression of IL-4, IL-17, and TGF-β were significantly decreased, but only Treg was increased in the dexamethasone treatment group ( P < 0.05 to P < 0.001). Carvacrol treatment during the sensitization period showed a more specific effect on Th1/Th2 imbalance in sensitized mice than dexamethasone, which may indicate the therapeutic potentials of carvacrol in disorders associated with Th1/Th2 imbalance such as asthma. |
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Keywords: | animal model asthma carvacrol cytokines flow cytometry |
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