Effect of resveratrol on the radiosensitivity of 5-FU in human breast cancer MCF-7 cells |
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Authors: | Shahin Aghamiri Ali Jafarpour Farshid Zandsalimi Mehran Aghemiri Mohsen Shoja |
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Affiliation: | 1. Student research committee, Department of medical biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Students' Scientific Research Center, Virology Division, Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran;3. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;4. Department of Medical Informatics, Tarbiat Modares University, Tehran, Iran;5. Faculty of Paramedicine, Semnan University of Medical Sciences, Semnan, Iran |
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Abstract: | The aim of this study was to assess the efficacy of resveratrol (Res) on radiosensitivity of 5-fluorouracil (5-FU) in the spheroid culture of MCF-7 breast cancer cell line using colony formation examination. Spheroids on day 9 with 300 µm diameters were treated with 20 µM resveratrol and/or 1 µM 5-FU for one volume doubling time (VDT) (42 hours) and then irradiated with 2 Gy gamma radiation (60Co) in various groups. Then the viability of the cells and clonogenic ability were acquired by blue dye exclusion and colony formation assay, respectively. The population doubling time in the monolayer culture and the VDT of spheroid culture was 22.48 0.23 hours and 42 0.63 hours respectively. None of the drugs and combination of them had any effect on the viability of cells. The combination treatment of 5-FU+Res+ radiation significantly reduced the colony formation ability of spheroid cells in comparison with each treatment alone. Our results indicated that resveratrol can significantly decrease colony number of breast cancer spheroid cells treated with 5-FU in combination with gamma-rays. Thus, resveratrol as a hypoxia-inducible factor-1-alpha inhibitor increased the radiosensitization of breast cancer spheroid cells. |
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Keywords: | 5-fluorouracil breast cancer radiation radiosensitivity resveratrol spheroid |
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