Nrf2 dependent antiaging effect of milk-derived bioactive peptide in old fibroblasts |
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Authors: | Naveen Kumar MSc PhD Scholar Srinu Reddi PhD Savita Devi MSc PhD Sanusi Bello Mada PhD Rajeev Kapila PhD Suman Kapila PhD |
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Institution: | 1. Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India;2. Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria |
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Abstract: | Prolonged passaging of primary fibroblast cells totally shapes the natural biological phenomena and leads to the appearance of features related to senescence. As a result, it is a good natural tool to delineate the molecular mechanism of cellular aging. The present investigation revealed the antiaging effect of milk-derived novel bioactive peptide (VLPVPQK). The peptide played an important role in downregulating apoptosis-related markers in late passages of cultured fibroblast cells. The peptide treatment to aged fibroblasts caused enhancement in cell migration, DNA integrity, and decrease in the lipid peroxidation, reactive oxygen species, nitric oxide production as well as pro-inflammatory cytokines, TNF-α and IL-6. Moreover, the peptide decreased the expression of apoptotic caspases, Bax, and senescence-associated β-galactosidase (SA-β-gal) proteins. The peptide pretreatment also enhanced the extracellular collagen protein and antiapoptotic, Bcl-xL. In addition, the peptide treatment reversed the senescence-related activity in fibroblasts by stimulating Nrf2 mediated antioxidative defense system and inhibiting the action of NFkB/p38MAPK signaling, similar to the commercially available inhibitor (SB203580) of p38MAPK. Thus, the peptide exhibits the antiaging effect in dermal fibroblast cells. |
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Keywords: | bioactive peptide p38MAP kinase prolonged serial passage senescence-associated secretory phenotype |
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