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Rn7SK small nuclear RNA is involved in cellular senescence |
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| Authors: | Maryam Musavi Fatemeh Kohram Mozhgan Abasi Zohreh Bolandi Mohammad Ajoudanian Samira Mohammadi-Yeganeh Seyed Mahmoud Hashemi Kazem Sharifi Hamid reza Fathi Hossein Ghanbarian |
| Institution: | 1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;2. Departments of Cell, Molecular, and Structural Biology, Miami University, Oxford, Ohio;3. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran;6. Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran;7. Department of Plastic and Reconstructive Surgery, Tehran University of Medical Science, Tehran, Islamic Republic of Iran;8. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran |
| Abstract: | Rn7SK is a conserved small nuclear noncoding RNA which its function in aging has not been studied. Recently, we have demonstrated that Rn7SK overexpression reduces cell viability and is significantly downregulated in stem cells, human tumor tissues, and cell lines. In this study, we analyzed the role of Rn7SK on senescence in adipose tissue-derived mesenchymal stem cells (AD-MSCs). For this purpose, Rn7SK expression was downregulated and upregulated via transfection and transduction, respectively, in AD-MSCs and subsequently, various distinct characteristics of senescence including cell viability, proliferation, colony formation, senescence-associated β galactosidase activity, and differentiation potency was analyzed. Our results demonstrated the transient knockdown of Rn7SK in MSCs leads to delayed senescence, while its overexpressions shows opposite effects. When osteogenic differentiation was started, however, they exhibited a greater differentiation potential than the original MSCs, suggesting a potential tool for stem cell-based regenerative medicine. |
| Keywords: | aging mesenchymal stem cells noncoding RNAs Rn7SK senescence |