Circular RNA circ_001350 regulates glioma cell proliferation,apoptosis, and metastatic properties by acting as a miRNA sponge |
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Authors: | Yang Liu Chunjie Ma Xiangying Qin Haiming Yu Lei Shen Haifeng Jin |
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Institution: | 1. Department of Neurosurgery, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China;2. Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China;3. Department of Medical Service, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China;4. Department of Anatomy, College of Basic Medicine, Qiqihar Medical University, Qiqihar, China |
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Abstract: | Glioma is an aggressive malignancy with increasing incidence and threatens people's health worldwide. Accumulating evidence revealed that circular RNAs (circRNAs) play important functions in cancers. A previous study demonstrated that circ_001350 was elevated in glioma tissue samples than nontumorous tissue specimens screened by high-throughput microarray. The level of circ_001350 in glioma tissue specimens and cell lines was detected by quantitative real-time polymerase chain reaction. The Fisher exact test was carried out to estimate the correlation of circ_001350 level with clinical characteristics. Cell proliferation, apoptosis, and motility abilities were detected using cell counting kit-8, clonogenic, flow cytometry, and transwell experiments, respectively. The potential target of circ_001350 was identified by the luciferase assay. circ_001350 level was significantly enhanced in glioma tissue specimens and cells. Further, elevated expression of circ_001350 was closely linked to patients' clinical severity. Knockdown of circ_001350 could inhibit cell proliferation and metastatic properties and increase apoptotic cells. circ_001350 could directly bind to miR-1236 and regulate its expression to exert oncogenic functions. Collectively, circ_001350 directly sponges miR-1236, thus contributing to malignant progression of glioma. |
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Keywords: | circ_001350 circular RNA glioma miR-1236 |
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