Formation and activation induction of primordial follicles using granulosa and cumulus cells conditioned media |
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| Authors: | Mohammad Jafari Atrabi Vahid Akbarinejad Ramazan Khanbabaee Azam Dalman Christiani Andrade Amorim Mustafa Najar-Asl Mojtaba Rezazadeh Valojerdi Rouhollah Fathi |
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| Affiliation: | 1. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran;2. Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran;3. Department of Biology, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran;4. Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium;5. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran;6. Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
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| Abstract: | Fertility preservation of prepubertal girls subjected to invasive cancer therapy necessitates defining protocols for activation of isolated primordial follicles. Granulosa (GCs) and cumulus cells (CCs) play pivotal role in oocyte development. Although GCs and CCs share some similarities, they differ in growth factors production. The current study was conducted to evaluate the effects of GCs, CCs and their conditioned media on mice primordial follicles activation. One-day-old mice ovaries were subjected to 6-day culture with base medium (BM), GC conditioned medium (GCCM), GC coculture (GCCC), CC conditioned medium (CCCM) or CC coculture (CCCC). Follicular growth and primordial to primary follicle transition was observed during 6-day culture, and follicular activation rate tended to be greater in GCCM than other groups (0.05 <P < 0.10). On Day 6, the expression of phosphatase and tensin homolog (PTEN) in GCCM group was lower than that in BM group (P = 0.020), the expression of phosphoinositide-3-kinase was higher in CCCC group than BM, GCCM and CCCM groups (P < 0.05), and the expression of connexin 37 was greater in the CCCM group as compared with BM, GCCC, and CCCC groups (P < 0.01). In conclusion, the current study showed that condition medium of GCs could enhance in vitro activation of primordial follicles, probably through downregulation of PTEN. |
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| Keywords: | condition medium granulosa cells Pi3k Pten primordial follicles activation |
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