IGF-1 and BMP-7 synergistically stimulate articular cartilage repairing in the rabbit knees by improving chondrogenic differentiation of bone-marrow mesenchymal stem cells |
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Authors: | Xian-Bo Peng Yuan Zhang Yue-Qiu Wang Qi He Qian Yu |
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Affiliation: | 1. Department of Joint Surgery & Sports Medicine, Qianfoshan Hospital of Shandong Province, Jinan, China;2. Department of Geriatric Neurology, Qianfoshan Hospital of Shandong Province, Jinan, China;3. Department of Joint Branch, Jining No. 2 People’s Hospital, Shandong Province, Jining, China;4. Department of Blood Transfusion, Shandong Provincial Hospital, Jinan, China |
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Abstract: | Knee injury is known as a frequently occurred damage related to sports, which may affect the function of cartilage. This study aims to explore whether Insulin-like growth factor 1 (IGF-1) and bone morphogenetic protein-7 (BMP-7)-modified bone-marrow mesenchymal stem cells (BMSCs) affect the repair of cartilage damage found in the knee. Primarily, BMSCs were treated with a series of pEGFP-C1, IGF-1, and BMP-7, followed by determination of IGF-1 and BMP-7 expression. A rabbit cartilage defect model was also established. Afterfward, cell morphology, viability, cartilage damage repair effect, and expression of collagen I and collagen II at the 6th and the 12th week were measured. BMSCs treated with pEGFP-C1/IGF-1, pEGFP-C1/BMP-7, and pEGFP-C1/BMP-7-IGF-1 exhibited elevated expression of BMP-7 and IGF-1. Besides, BMSCs in the P10 generation displayed decreased cell proliferation. Moreover, BMSCs treated with IGF-1, BMP-7, and IGF-1-BMP-7 showed reduced histological score and collagen I expression while elevated collagen II expression, as well as better repair effect, especially in those treated with IGF-1-BMP-7. Collectively, these results demonstrated a synergistic effect of IGF-1 and BMP-7 on the BMSC chondrogenic differentiation on the articular cartilage damage repair in the rabbit knees, highlighting its therapeutic potential for the treatment of articular cartilage damage. |
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Keywords: | bone morphogenetic protein-7 bone-marrow mesenchymal stem cells cartilage damage gene modification Insulin-like growth factor 1 |
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