MicroRNA-628-5p inhibits cell proliferation in glioma by targeting DDX59 |
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| Authors: | Peng Xie Yan Wang Yuanmei Liao Qiu Han Zhichao Qiu Yinbing Chen Xiaohua Zuo |
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| Institution: | 1. Department of Neurosurgery, The Second People's Hospital of Huai'an, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China;2. Department of Medicine Laboratory, The Second People's Hospital of Lianyungang, Lianyungang, Jiangsu, China;3. Department of Medical Technology, Gannan Healthcare Vocational College, Ganzhou, China;4. Department of Neurology, The Second People's Hospital of Huai'an, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China;5. Department of Neurosurgery, BenQ Medical Center, The Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China;6. Department of Orthopedic Surgery, The Affiliated Haian Hospital of Nantong University, Haian, Jiangsu, China;7. Department of Pain Management, The Second People's Hospital of Huai'an, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China |
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| Abstract: | Recent study has reported that microRNA-628-5p (miR-628-5p) is involved in the development of epithelial ovarian cancer; however, the mechanisms of miR-628-5p in glioma remain unclear. In this study, we explored the potential biological roles of miR-628-5p in glioma. First, we found that miR-628-5p was decreased in the tissues and cells (U87 and T98) of glioma. Second, overexpressing miR-628-5p reduced the ability of glioma cells' proliferation and induced glioma cells' cycle arrest in G1. Then, we found that miR-628-5p directly bound to the 3′-untranslated region of DDX59 and decreased the protein level of DDX59. The decrease of DDX59 was found to lead to the decrease of p-AKT. Mechanistic studies revealed that restoring the expression of DDX59 alleviated miR-628-5p-induced inhibition of proliferation of glioma. These findings suggest that the miR-628-5p/DDX59 axis has a key role in the development of glioma, and miR-628-5p might be a new therapeutic target against glioma. |
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| Keywords: | cell cycle arrest DDX59 glioma miR-628-5p proliferation |
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