长江华溪蟹精子形成的研究

1994年9-11月,对采自安徽省宁国县的长江华溪蟹(Sinopotamon yangtsekiense),利用透射电镜技术,并结合细胞化学方法,研究了其精子形成过程。结果显示:早期精细胞圆形,胞质丰富,内含大量内质同小泡及线粒体。核也为圆形,较小。精细胞开始分化,细胞膨胀为长椭圆形,核质重新分布,分别移向细胞的两端。精子的顶体由高尔基体产生,其过程为:高尔基体分泌产生囊泡,继而形成原顶体囊,进一步发育成顶体囊,最后形成顶体。在顶体囊与核之间有膜复合体。中心粒位于核内面凹陷处。细胞化学反应显示,核杯为Feulgen阳性,顶体为PAS阳性。  

STUDIES ON SPERMIOGENESIS OF A FRESHWATER CRABSINOPOTAMON YANGTSEKIENSE (CRUSTACEA DECAPODA)

From September to November in 1994, the freshwater crab Sinopotamonyangtsekiense collected from Anhui Province were observed in detail with transmissionelectron microscope (TEM) and cytochemical methods. The early spermatid is smallwith an oval shaped nucleus. The spermahd began to differentiate, and changed intolonger elliptical shape, nuclear-plasm redistributed and moved towards two ends of thecell. The acrosome of sperm derived from Golgi body. The vesicles which producedfrom Golgi body aggregated to form a large proacrosomal vesicle. The proacrosomalvesicle further develop into acrosomal vesicle. The nuclear membrare changes into acup-like shape and surrounds the acrosomal vesicle, between the two parts, and thena layer of membrane complex appears. The nuclear cup was positive and acrosomePAS positive.