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Fast manipulation of cellular cAMP level by light in vivo |
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| Authors: | Schröder-Lang Saskia Schwärzel Martin Seifert Reinhard Strünker Timo Kateriya Suneel Looser Jens Watanabe Masakatsu Kaupp U Benjamin Hegemann Peter Nagel Georg |
| Institution: | Max-Planck-Institut für Biophysik, 60439 Frankfurt, Germany. |
| Abstract: | The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells. |
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