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Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol |
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| Authors: | Dong-Wook?Han,Hak?Hee?Kim,Mi?Hee?Lee,Hyun?Sook?Baek,Kwon-Yong?Lee,Suong-Hyu?Hyon,Jong-Chul?Park author-information" > author-information__contact u-icon-before" > mailto:parkjc@yumc.yonsei.ac.kr" title=" parkjc@yumc.yonsei.ac.kr" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
| Affiliation: | (1) Department of Medical Engineering, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea;(2) Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, 134 Shinchon-dong, Seodaemun-gu, Seoul, 120-752, Korea;(3) Bioengineering Research Center, Department of Mechanical Engineering, Sejong University, 98 Gunja-dong, Gwangjin-gu, Seoul, 143-747, Korea;(4) Institute for Frontier Medical Sciences, Kyoto University, Shogoin, Sakyo-ku Kyoto, 606-8507, Japan |
| Abstract: | Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml−1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering. |
| Keywords: | green tea polyphenol multiple freeze/thaw cycles osteoblastic cells oxidative stress |
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