Functional Analysis of B144/LST1: A Gene in the Tumor Necrosis Factor Cluster That Induces Formation of Long Filopodia in Eukaryotic Cells |
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Authors: | Arumugham Raghunathan Ramou Sivakamasundari Joseph Wolenski Ranjana Poddar Sherman M. Weissman |
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Affiliation: | a Boyer Center for Molecular Medicine, Room 333, Department of Genetics, Yale School of Medicine, 295 Congress Avenue, New Haven, Connecticut, 06536;b MCDB Department, Yale University, 06510 |
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Abstract: | B144/LST1 is a gene encoded in the human major histocompatibility complex that produces multiple forms of alternatively spliced mRNA and encodes peptides fewer than 100 amino acids in length. B144/LST1 is strongly expressed in dendritic cells. Transfection of B144/LST1 into a variety of cells induces morphologic changes including the production of long, thin filopodia differing from those seen on transfection of a dominant active CDC42 gene. The structures are dynamically rearranging and sometimes connect one cell with another. The full effect of B144/LST1 protein on cell morphology requires the retention of at least one of the four cysteines of the peptide plus the presence of a hydrophobic segment in the protein, but requires only one of the two coding regions present in the terminal 3′ exons. |
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Keywords: | B144 LST1 cytoskeletal reorganization microspikes filopodia |
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