A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced <Emphasis Type="Italic">Renilla</Emphasis>luciferase-antigen fusion proteins期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced <Emphasis Type="Italic">Renilla</Emphasis>luciferase-antigen fusion proteins

Authors:	Email author" target="_blank">Peter?D?Burbelo Email author Radoslav?Goldman Thomas?L?Mattson

Institution:	(1) Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, D.C 20057, USA

Abstract:	Background Assays detecting human antigen-specific antibodies are medically useful. However, the usefulness of existing simple immunoassay formats is limited by technical considerations such as sera antibodies to contaminants in insufficiently pure antigen, a problem likely exacerbated when antigen panels are screened to obtain clinically useful data.

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