The role of DNA base excision repair in filamentation in Escherichia coli K-12 adhered to epithelial HEp-2 cells |
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Authors: | Suelen B Costa Ana Carolina C Campos Ana Claudia M Pereira Ana Luiza de Mattos-Guaraldi Raphael Hirata Júnior Ana Cláudia P Rosa Lídia M B O Asad |
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Institution: | 1. Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil 2. Departamento de Biofísica e Biometria, IBRAG, UERJ, Av. 28 de Setembro, 87–Fundos, 4o andar, Vila Isabel, Rio de Janeiro, CEP: 20.551-030, Brazil
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Abstract: | Base excision repair (BER) is dedicated to the repair of oxidative DNA damage caused by reactive oxygen species generated
by chemical and physical agents or by metabolism which can react with DNA and cause a variety of mutations. Epithelial cells
are typically the first type of host cell to come into contact with potential microbial invaders. In this work, we have evaluated
whether the adherence to human epithelial cells causes DNA damage and associated filamentation. Experiments concerning adherence
to HEp-2 cells were carried out with mutants deficient in BER that were derived from Escherichia coli K-12. Since the removal of mannose during bacterial interaction with HEp-2 cells allows adhesion through mannose-sensitive
adhesins, the experiments were also performed in the presence and the absence of mannose. Our results showed enhanced filamentation
for the single xth (BW9091) and triple xth nfo nth (BW535) mutants in adherence assays with HEp-2 cells performed without d-mannose. The increased filamentation growth was inhibited by complementation of BER mutants with a wild type xth gene. Moreover, we measured SOS induction of bacteria adhered to HEp-2 cells in the presence and absence of d-mannose through of SOS-chromotest assay and we observed a higher β-galactosidase expression in the absence of mannose. In
this context, data showed evidence that bacterial attachment to HEp-2 epithelial surfaces can generate DNA lesions and SOS
induction. |
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