Effects of serum deprivation on expression of proteolytic-related genes in chick myotube cultures |
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Authors: | Nakashima Kazuki Yamazaki Makoto Abe Hiroyuki |
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Institution: | Nutrient Function Laboratory, Department of Animal Physiology and Nutrition, National Institute of Livestock and Grassland Science, Ikenodai, Tsukuba, Ibaraki, Japan. kaznaka@affrc.go.jp |
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Abstract: | We previously reported that serum deprivation stimulates myofibrillar proteolysis in chick myotubes. In the present study, we examined the effect of serum deprivation on expression of the proteolytic-related genes (ubiquitin, proteasome, calpains, and cathepsin B) by real-time PCR of cDNA in chick myotubes. Myotubes were incubated with serum-free medium for 24 h. Ubiquitin and proteasome subunits (C1 and C2) and calpains (m-, mu-, and p94/calpain-3) but not cathepsin B mRNA expression were increased by serum deprivation. These results indicate that serum deprivation stimulates ubiquitin-proteasome and calpain proteolytic pathways, resulting in an increase in myofibrillar proteolysis in chick myotubes. |
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