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内蒙古中东部草原区克氏针茅形态特征和RAPD遗传分化的相关性研究
引用本文:王金龙,高玉葆,赵念席,刘景玲,林枫,宋涛.内蒙古中东部草原区克氏针茅形态特征和RAPD遗传分化的相关性研究[J].植物研究,2006,26(6):708-714.
作者姓名:王金龙  高玉葆  赵念席  刘景玲  林枫  宋涛
作者单位:南开大学生命科学学院,天津,300071
基金项目:国家重点基础研究发展计划(973计划)
摘    要:以分布在内蒙古锡林郭勒盟东部草甸草原、中部典型草原和中西部荒漠化草原的4个克氏针茅种群为研究对象,采用形态学标记和RAPD分子标记相结合的方法进行遗传分化研究。结果表明:(1)无论是用形态学数据所得欧氏遗传距离矩阵还是用RAPD所得无偏差的Nei’s遗传距离矩阵,与种群分布的地理距离之间均不存在显著的相关关系,说明克氏针茅种群遗传分化受自然选择的影响。(2)种群之间存在显著的形态分化和遗传分化(p<0.05)。(3)对形态学数据所得欧氏遗传距离矩阵和RAPD所得Nei’s无偏差遗传距离矩阵进行Mantel检验所得结果不显著,表明对克氏针茅形态分化和遗传分化起主要作用的选择力是不完全相同的。

关 键 词:内蒙古草原  克氏针茅  遗传分化  RAPD  形态特征
收稿时间:2005-11-16
修稿时间:2005年11月16

Morphological and RAPD genetic differentiation of Stipa krylovii in middle and eastern Inner Mongolia steppe
WANG Jin-Long,GAO Yu-Bao,ZHAO Nian-Xi,LIU Jing-Ling,LIN Feng,SONG Tao.Morphological and RAPD genetic differentiation of Stipa krylovii in middle and eastern Inner Mongolia steppe[J].Bulletin of Botanical Research,2006,26(6):708-714.
Authors:WANG Jin-Long  GAO Yu-Bao  ZHAO Nian-Xi  LIU Jing-Ling  LIN Feng  SONG Tao
Institution:College of Life Science, Nankai University, Tianjin 300071
Abstract:Genetic differentiation of four Stipa krylovii populations collected from meadow steppe,typical steppe and desert steppe in middle and eastern Inner Mongolia steppe,was studied with a combination of morphological and RAPD markers.(1) Neither Euclidean's distance matrix based on morphological data nor Nei's unbiased genetic distance matrix had any significant relationships with geographic distance matrix in the region.This indicated that genetic differentiation of Stipa krylovii populations was affected mainly by natural selection.(2) One way ANOVA and AMOVA showed that there were significant morphological divergence and genetic differentiation.(3) The relationship was not significant between Euclidean's distance and Nei's unbiased genetic distance by Mantel test,suggesting that the evolutionary force was not identical on morphological divergence and genetic differentiation.
Keywords:Inner Mongolia steppe  Stipa krylovii Roshev    genetic differentiation  RAPD  morphological traits
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