北京地区非洲菊叶斑病病原菌鉴定

采用离体叶片接种致病性测定、形态学观察和分子鉴定方法对非洲菊叶斑病病原进行鉴定。从北京某花卉基地采集非洲菊叶斑病样,分离获得22个真菌分离物,分别记作GL1-GL22。经过致病性试验证实,分离物GL14和GL22为非洲菊叶斑病病原菌。经形态学鉴定,GL14为细极链格孢Alternaria tenuissima,GL22为链格孢Alternaria alternata。采用真菌通用引物对GL14和GL22 rDNA的ITS区进行PCR扩增,并将测序结果在GenBank中进行同源性比对分析,分子鉴定与形态学鉴定结果一致。

Isolation and identification of pathogens of gerbera leaf spot in Beijing

The pathogens of gerbera spot disease were identified on the basis of pathogenic test, as well as morphological characters and molecular identification. Diseased gerbera samples were collected from a gerbera plantation in Beijing. Twenty-two isolations of possible pathogenic fungi were isolated from leaves of gerbera plants. The results showed that GL14 and GL22 were the pathogens of gerbera spot disease. Morphological identification showed that GL14 was Alternaria tenuissima and GL22 Alternaria alternata. ITS sequences of the isolations GL14 and GL22 were amplified by PCR technique with universal primers of fungi, and the sequences were analyzed by comparing with those in GenBank. The molecular identification indicated the same results as morphology identification.