二甲基亚砜作用下重组CHO细胞胞内乙型肝炎表面抗原的定位

在培养环境中添加二甲基亚砜 (DMSO) 能分别提高重组 CHO 细胞乙型肝炎表面抗原（HBsAg）的产量和比生产速率70%和3.2倍以上, 但同时发现胞内HBsAg 的积累量是对照组的7.2倍。为了分析胞内HBsAg 积累的区域，采用电镜技术分析后发现，经DMSO处理后的CHO细胞胞内出现了很多的扩张区域，这些扩张区域分布整个胞浆，有的扩张区域已经侵蚀到细胞核上，而对照组未发现明显的扩张区域。进一步利用免疫电镜技术分析后发现，经DMSO处理后的细胞胞内大量积累的HBsAg主要分布在这些扩张区域中，同时发现在细胞核膜上也有分布，这可能是由于扩张区域侵蚀细胞核造成的。以上工作有助于揭示在DMSO作用下重组CHO细胞胞内HBsAg大量积累的机制。

Localization of hepatitis B surface antigen within recombinant CHO cells in response to dimethyl sulfoxide

The intracellular hepatitis B surface antigen (HBsAg) content per cell was increased by 7.2-fold in the culture with 1.5% dimethyl sulfoxide (DMSO) compared with that in the control without DMSO, while the extracellular HBsAg production and specific productivity were only improved by 70% and 3.2-fold, respectively. Electron microscope has been employed to reveal large dilated structures within recombinant CHO cells in the presence DMSO. The dilated structures have a distribution within whole cytoplasm, and some dilated areas were engulfed in the nucleus. These large, dilated structures were not observed in the control. Immunogold labeling was used to discover the accumulated HBsAg was localized within these dilated areas, and some HBsAg-specific labels were detected in the nucleus membrane, owing to the encroachment of the dilated areas upon nucleus. This work could help to reveal the mechanism of intracellular HBsAg accumulation in the presence of DMSO.