Human interleukin-1 receptor antagonist. High yield expression in E. coli and examination of cysteine residues. |
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| Authors: | A Steinkasserer R Solari H R Mott R T Aplin C C Robinson A C Willis R B Sim |
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| Institution: | Department of Biochemistry, University of Oxford, UK. |
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| Abstract: | The human IL-1 receptor antagonist (IL-1ra) was produced in a high yield E. coli expression system, and was purified in a rapid two-step purification. This recombinant IL-1ra molecule possessed full binding activity to the IL-1 receptor (type I) and totally inhibited IL-1-induced PGE2 production by human dermal fibroblasts. Radioalkylation and analysis of V8-derived IL-1ra peptides indicate that the four cysteines present in the IL-1ra are not disulphide-linked. |
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