A Highly Sensitive Enzyme Catalytic Method for the Detection of Ethanol Based on Resonance Scattering Effect of Gold Particles |
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| Authors: | Yanghe Luo Pengfei Wang Tingsheng Li Jianniao Tian Aihui Liang Zhiliang Jiang |
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| Affiliation: | 1. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education; Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin, 541004, China
2. Province Key Construction Laboratory of Characteristic Resources Research and Development of East Guangxi, Hezhou University, Hezhou, 542800, China
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| Abstract: | In pH 8.4 Tris–HCl buffer solutions, alcohol dehydrogenase catalyzed the reaction between ethanol and nicotinamide adenine dinucleotide to produce acetaldehyde. In the medium of HCl, acetaldehyde reduced HAuCl4 to form gold particles that exhibited a strong resonance scattering (RS) peak at 600 nm. The RS peak increased with ethanol concentration. The increased RS intensity at 600 nm (ΔI 600 nm) was proportional to the ethanol concentration (C) from 0.068 to 10.2 mmol/L, with a regression equation of ΔI 600 nm?=?35.59?C?+?16.1, and a detection limit (3σ) of 3.2 μmol/L. This proposed method was applied to detect ethanol in saliva and plant cell culture medium samples, with satisfactory results. |
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