Functionalized Plasmonic Anisotropic Nanocrystals for Multimodal Imaging of Cancer Cells |
| |
Authors: | Atcha Kopwitthaya Ken-Tye Yong Andrey Kuzmin Wing-Cheung Law Artem Pliss Lisa A Vathy Indrajit Roy Paras N Prasad |
| |
Institution: | 1. Institute for Laser, Photonics and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY, 14260-4200, USA 2. School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, 639798, Singapore 3. Department of Chemistry, University of Delhi, Delhi, 110 007, India
|
| |
Abstract: | Nanoparticles internalized by cells are valuable probes for bioimaging. In particular, nanoparticles can be detected in “biological transmission window,” i.e., near infrared region. Here, we report a preparation of biotargeting diethylthiatricarbocyanine iodide (DTTC)-functionalized gold nanorods, utilized for detection of malignant cells. These biotargeting DTTC-functionalized gold nanorods are efficiently internalized into cultured cells and can serve as probes for surface-enhanced Raman scattering (SERS) and dark-field imaging. The robust SERS signal from malignant cells has clearly demonstrated a signature peak of DTTC in the presence of our formulation. A short acquisition time, we used in this experiment, is able to exclude bulk of Raman signal from natural cellular constituents. This signature peak will be a key of identifying cancer due to cancer-specific property of biotargeted molecule. The results are leading to promising real-time cancer detection. In addition, these multimodal probes demonstrated low toxicity in cell viability studies which enables a broad range of multiplex imaging applications. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|