Use of nucleofection to efficiently transfect primary rabbit lacrimal gland acinar cells |
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Authors: | Janette Contreras Pang-Yu Hsueh Hua Pei Sarah F. Hamm-Alvarez |
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Affiliation: | (1) Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Los Angeles, CA 90033, USA; |
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Abstract: | Lacrimal gland acinar cells are an important cell type to study due to their role in production and release of tear proteins, a function essential for ocular surface integrity and normal visual acuity. However, mechanistic studies are often limited by problems with transfection using either plasmid DNA or siRNA. Although various gene delivery methods are available, many have been unproductive due to consistently low transfection efficiencies. We have developed a method using nucleofection that can result in 50% transfection efficiency and 60% knockdown efficiency for plasmid DNA and siRNA, respectively. These results are vastly improved relative to previous studies, demonstrating that nucleofection offers an efficient transfection technique for primary lacrimal gland acinar cells. |
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Keywords: | Transfection Nucleofection Primary epithelial cells Acinar cells Lacrimal gland |
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