Evaluation of immunostimulatory effect of the arrowroot (<Emphasis Type="Italic">Maranta arundinacea</Emphasis>. L) in vitro and in vivo |
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| Authors: | Ika Dyah Kumalasari Eni Harmayani Lily Arsanti Lestari Sri Raharjo Widya Asmara Kosuke Nishi Takuya Sugahara |
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| Institution: | (1) Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama Ehime, 790-8566, Japan;(2) Faculty of Agricultural Technology, Gadjah Mada University, Jl. Socio Yusticia, Bulaksumur, Yogyakarta, 55281, Indonesia;(3) Faculty of Medicine, Gadjah Mada University, Jl. Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia;(4) Faculty of Veterinary Medicine, Gadjah Mada University, Jl. Fauna No. 2, Karang Malang, Yogyakarta, 55281, Indonesia;(5) South Ehime Fisheries Research Center, Ehime University, 1289-1 Funakoshi, Ainan Ehime, 789-4292, Japan; |
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| Abstract: | Arrowroot (Maranta arundinacea. L) is an underutilized local crop potentially to be developed as carbohydrate source and functional food in Indonesia. The
objectives of this research are to evaluate the immunostimulatory effects of arrowroot extracts in vitro by using animal cell
culture techniques, and in vivo by using BALB/c mice. The arrowroot tuber extracts were prepared by heat-treatment at 121 °C
for 20 min in distilled water. The IgM production stimulatory activity of arrowroot tuber extracts against human hybridoma
HB4C5 cells and mouse splenocytes was assessed. The result indicated that the arrowroot tuber extract stimulated IgM production
by HB4C5 cells and immunoglobulin (IgG, IgA and IgM) production by splenocytes in vitro. In addition, the arrowroot tuber
extracts strongly enhanced interferon γ production by splenocytes. In vivo study indicated that the diet containing arrowroot
extracts increased the serum IgG, IgA and IgM levels in mice. These results revealed that the arrowroot tuber extracts have
immunostimulatory effects in vivo as well as in vitro. |
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| Keywords: | Arrowroot (Maranta arundinacea) Immunoglobulin Immunostimulatory effect Interferon γ Splenocytes |
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