Protein dynamics during seed germination under copper stress in Arabidopsis over-expressing <Emphasis Type="Italic">Potentilla superoxide dismutase</Emphasis> |
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Authors: | Tejpal?Gill Vivek?Dogra Sanjay?Kumar Paramvir?Singh?Ahuja Email author" target="_blank">Yelam?SreenivasuluEmail author |
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Institution: | (1) Biotechnology Division, Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research), Palampur, 176061, Himachal Pradesh, India; |
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Abstract: | Copper (Cu), though an essential micronutrient for plants, poses toxicity at higher concentrations possibly by inducing oxidative
stress. With the background that enzyme superoxide dismutase (SOD) ameliorates oxidative stress, the present work focused
on understanding physiological and proteomic response of Arabidopsis seeds constitutively over-expressing copper–zinc SOD of Potentilla atrosanguinea (PaSOD) during germination in response to varied concentrations of copper sulphate (Cu stress). Transgenics showed higher germination
percentage and required less “mean time to germination” under Cu-stress. In response to Cu stress, 39 differentially expressed
protein spots were detected by 2-D electrophoresis in proteins of germinating wild type (WT) and transgenic seeds, of which
14 spots appeared exclusively in transgenics. Among the rest 25 protein spots, 14 showed down-regulation, one showed up-regulation,
and 10 spots disappeared. MALDI-TOF and subsequent peptide mass fingerprinting analysis revealed that the down-regulated proteins
in transgenics were related to oxidative stress, detoxification, germination, intermediary metabolism and regulatory proteins.
Up-regulated proteins in WT and down-regulated proteins in transgenic during Cu stress were the same. Changes in key proteins,
vis-à-vis alleviation of oxidative stress in transgenic Arabidopsis over-expressing PaSOD possibly alleviated toxicity of Cu-induced stress during seed germination, resulting in higher germination rate and germination
percentage. |
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Keywords: | |
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