Use of immobilized bacteria to treat industrial wastewater containing a chlorinated pyridinol |
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| Authors: | Y Feng K D Racke J-M Bollag |
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| Institution: | (1) Laboratory of Soil Biochemistry, Center for Bioremediation and Detoxification, 129 Land and Water Building, The Pennsylvania State University, University Park, PA 16802, USA. Fax :+1 814 865–7836 e-mail : jmbollag@psu.edu, US;(2) Environmental Chemistry Laboratory, DowElanco, Indianapolis, IN 46268, USA, IN |
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| Abstract: | Pseudomonas sp. strain M285 immobilized on diatomaceous earth beads was used to remove 3,5,6-trichloro-2-pyridinol (TCP) from industrial
wastewater. Batch studies showed that immobilized Pseudomonas sp. strain M285 mineralized 2,6-14C]TCP rapidly; about 75% of the initial radioactivity was recovered as 14CO2. Transformation of TCP was inhibited by high concentrations of salt, and addition of osmoprotectants (proline and betaine
at 1 mM) did not reduce the adverse effect of salt. TCP-containing wastewater (60–140 mg/l) was passed through columns containing
immobilized Pseudomonas sp. strain M285 at increasing flow rates and increasing TCP concentrations; TCP removal of 80%–100% was achieved. Addition
of nutrients, such as glucose and yeast extract, retarded TCP degradation. Growing cell cultures were found to be better inocula
for immobilization than resting cells.
Received: 5 February 1996 / Received last revision: 12 August 1996 / Accepted: 24 August 1996 |
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