Leptospira interrogans requires a functional heme oxygenase to scavenge iron from hemoglobin |
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Authors: | Murray Gerald L Ellis Katherine M Lo Miranda Adler Ben |
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Institution: | aAustralian Bacterial Pathogenesis Program, Department of Microbiology, Monash University, VIC 3800, Australia;bAustralian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, VIC 3800, Australia |
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Abstract: | The transposon TnSC189 was used to construct a mutant in the putative heme oxygenase gene hemO (LB186) of Leptospira interrogans. Unlike its parent strain, the mutant grew poorly in medium in which hemoglobin was the sole iron source. The putative heme oxygenase was over expressed in a His-tagged form, purified and was demonstrated to degrade heme in vitro. Unexpectedly, it was also found that the L. interrogans growth rate was significantly increased when medium was supplemented with hemoglobin, but only if ferrous iron sources were absent. This result was mirrored in the expression of some iron-related genes and suggests the presence of regulatory mechanisms detecting Fe2+ and hemoglobin. This is the first demonstration of a functional heme oxygenase from a spirochete. |
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Keywords: | Leptospira interrogans Heme oxygenase Heme Iron Gene regulation |
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