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Quantitative Assessment of the Tetracycline Resistance Gene Pool in Cheese Samples by Real-Time TaqMan PCR
Authors:Michele Y. Manuzon   Scott E. Hanna   Hongliang Luo   Zhongtang Yu   W. James Harper     Hua H. Wang
Abstract:A TaqMan real-time PCR assay was developed to quantify the tetS gene pool present in retail cheeses. This protocol offers a rapid, specific, sensitive, and culture-independent method for assessing antibiotic resistance genes in food samples rich in fats and proteins.
Keywords:
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