Cytoskeletal association of muscle-specific mRNAs in differentiating L6 rat myoblasts |
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| Authors: | T Bagchi D E Larson B H Sells |
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| Institution: | 1. Lipids and Atherosclerosis Unit, IMIBIC/Reina Sofia University Hospital/University of Córdoba, Córdoba, Spain;2. CIBER Fisiopatología de la Obesidad y la Nutrición (CIBEROBN), Instituto de Salud Carlos III, Madrid, Spain;3. Biochemical Laboratory, Reina Sofia University Hospital, Córdoba, Spain;4. Nutrition and Genomics Laboratory, J.M,-US Department of Agriculture Human Nutrition Research Center on Aging, Tufts University, Boston, MA, USA;5. IMDEA-Food and CNIC, Madrid, Spain;6. Biomedical Research Laboratory, Endocrinology Department, Hospital Virgen de la Victoria, Malaga, Spain;2. Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, Western University, London, Ont.;3. Ivey Eye Institute, St. Joseph''s Health Care, London, Ont.;4. Schulich School of Medicine and Dentistry, Western University, London, Ont.;1. Department of Medicine, University of California San Diego, San Diego, CA;2. Department of Epidemiology & Prevention, Wake Forest School of Medicine, Winston-Salem NC;3. Department of Family Medicine and Public Health, University of California San Diego, San Diego, CA;4. Department of Public Health Services, University of California Davis, Davis, CA;5. Atlanta VA Medical Center, Decatur, GA and Division of Endocrinology and Metabolism, Department of Medicine, Emory University School of Medicine, Atlanta, GA;6. The Miriam Hospital Centers for Behavioral and Preventive Medicine at the Warren Alpert Medical School, Brown University, Providence, RI;7. School of Nursing, University of Washington, Seattle, WA |
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| Abstract: | The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. Polysomal mRNPs were predominantly associated with the cytoskeletal framework and free mRNPs were present in both soluble and cytoskeletal fractions. An examination of the distribution of specific mRNAs in the polysomal and free mRNP populations of both cytoplasmic fractions revealed differences in the pattern of their distribution. It is further demonstrated that in the L6 rat myoblast system, ribosomes and mRNA (or mRNP) are not associated with the microfilaments, unlike in other systems studied. |
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