NEIL1 excises 3' end proximal oxidative DNA lesions resistant to cleavage by NTH1 and OGG1 |
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Authors: | Parsons Jason L Zharkov Dmitry O Dianov Grigory L |
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Institution: | MRC Radiation and Genome Stability Unit Harwell, Oxfordshire, OX11 0RD, UK. |
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Abstract: | Base excision repair is the major pathway for the repair of oxidative DNA damage in human cells that is initiated by a damage-specific DNA glycosylase. In human cells, the major DNA glycosylases for the excision of oxidative base damage are OGG1 and NTH1 that excise 8-oxoguanine and oxidative pyrimidines, respectively. We find that both enzymes have limited activity on DNA lesions located in the vicinity of the 3′ end of a DNA single-strand break, suggesting that other enzymes are involved in the processing of such lesions. In this study, we identify and characterize NEIL1 as a major DNA glycosylase that excises oxidative base damage located in close proximity to the 3′ end of a DNA single-strand break. |
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