Size of poly (A) +-mRNA in Saccharomyces cerevisiae polysomes. |
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| Authors: | Z E Mateeva A A Hadjiolov |
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| Affiliation: | Institute of Molecular Biology, Bulgarian Academy of Sciences 1113 Sofia, Bulgaria |
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| Abstract: | The size of poly (A) +-mRNA in different classes of yeast polysomes is estimated. The average molecular weight of long-term labelled polysomal poly (A) +-mRNA is about 0,65 x 10(6) daltons. Approximately 60% of the poly (A) +-mRNA polynucleotide chains located at the 5' end, are unprotected by ribosomes and degraded by nucleases upon incubation of cell lysates, to yield a population of poly (A) +-mRNA with an average molecular weight of 0,25 x 10(6) daltons. |
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