| 羊胎免疫调节因子制造工艺研究 |
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| 引用本文： | 牛荣,魏泓.羊胎免疫调节因子制造工艺研究[J].氨基酸和生物资源,2006,28(3):60-61,75. |
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| 作者姓名： | 牛荣 魏泓 |
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| 作者单位： | 第三军医大学基础部动物学教研室,重庆,400038;第三军医大学基础部动物学教研室,重庆,400038 |
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| 摘 要： | 目的:优选出羊胎免疫调节因子提取的最佳工艺。方法:采用正交实验设计L8(27),以淋巴细胞转化实验为考察指标进行实验,对提取过程中的6个指标进行了优化,确定了最佳提取工艺。结果:羊胎免疫调节因子最佳提取工艺为A1B2C2D1E2F2,即2月龄内羊胎粉碎10 m in,加1:4双蒸水常温下8000 r.m in-1离心后10K超滤膜超滤即得。
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| 关 键 词： | 羊胎免疫调节因子 提取工艺 正交设计 |
| 文章编号： | 1006-8376（2006）03-0060-02 |
| 收稿时间： | 03 8 2006 12:00AM |
| 修稿时间： | 2006-03-08 |
Study on Optimum Extraction Process for Goat Embryo Immunoregulatory Factor (GEIF) |
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| NIU Rong,WEI Hong.Study on Optimum Extraction Process for Goat Embryo Immunoregulatory Factor (GEIF)[J].Amino Acids & Biotic Resources,2006,28(3):60-61,75. |
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| Authors: | NIU Rong WEI Hong |
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| Abstract: | To study the optimum extraction process for goat embryo immunoregulatory factor(GEIF),the lymphocyte transformation was investiqated.There were 6 indexes in the extraction process by orthogonal design,and then the optimum extraction process was confirmed.The optimum extraction process was A1B2C2D1E2F2,namely,the goat embryo's month age was younger than 2,crushed 10min,added double-distilled water1:4(v/v),normal temperature,centrifuged with 8000g,and obtained 10K molecular weight by ultrafilter. |
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| Keywords: | goat embryo immunoregulatory factor(GEIF) extraction process orthogonal design |
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