Beet necrotic yellow vein virus: different isolates are serologically similar but differ in RNA composition |
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| Authors: | MARIA KUSZALA,VÉ RONIQUE ZIEGLER&dagger ,S. BOUZOUBAA&dagger ,K. RICHARDS&dagger ,C. PUTZ,H. GUILLEY&dagger ,G. JONARD&dagger |
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| Affiliation: | *Station de Pathologie Végétale, Institut National de la Recherche Agronomique, 28 rue de Herrlisheim, 68021 Colmar Cédex, France;†Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire, 15 rue Descartes, 67084 Strasbourg Cédex, France |
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| Abstract: | Twelve isolates of beet necrotic yellow vein virus, agent of rhizomania in sugar beet, have been obtained from five European countries and the USA. The isolates were cloned and multiplied in the local lesion host Chenopodium quinoa. By ELISA, a close serological relationship was observed among all the isolates. The isolates differed, however, in the number of viral RNA species present (2, 3 or 4) and in the lengths of RNAs 3 and 4, the two smallest RNAs. A comparison of the symptoms induced by the different isolates in C. quinoa revealed a correlation between the presence of a full-length RNA 3 (about 1850 nucleotides) and the appearance of severe chlorotic local lesions. |
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