| 亚甲基兰法检测人白介素—2（rhIL—2）活性 |
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| 引用本文： | 郑晓丽,杨倩.亚甲基兰法检测人白介素—2（rhIL—2）活性[J].微生物学杂志,2000,20(3):57-59. |
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| 作者姓名： | 郑晓丽 杨倩 |
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| 作者单位： | 长春生物制品研究所!130062(郑晓丽，隋宝真，李英华，孙凤至)，长春长生基因药业股份有限公司(杨倩) |
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| 摘 要： | 用亚甲基兰法、3 H TdR掺入法和MTT法 3种方法对 2 0批检品重组人白介素 2 (rhIL 2 )活性进行检测 ,结果差异不显著 ( p >0 .0 5)。与3 H -TdR掺入法和MTT法相比较 ,亚甲基兰法具有经济、快捷、对操作者无损害和对环境无污染等特点 ,是一种值得采用的重组人白介素 2活性检测方法 ,适合重组人白介素 2生产过程中中间品的质量监控。
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| 关 键 词： | 人重组白介素-2 活性检测 染色法 亚甲基兰 |
METHYLENE BLUE METHOD TO DETECT BIOLOGICAL ACTIVITY OF RECOMBINANT HUMAN INTERLEUKIN 2 (rhil-2) |
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| Zheng Xiaoli et al..METHYLENE BLUE METHOD TO DETECT BIOLOGICAL ACTIVITY OF RECOMBINANT HUMAN INTERLEUKIN 2 (rhil-2)[J].Journal of Microbiology,2000,20(3):57-59. |
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| Authors: | Zheng Xiaoli |
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| Institution: | Changchun Inst. Biological Products. Changchun 130062 |
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| Abstract: | A new detection of biological activity of recombinant human interleukin 2 (rhIL-2) methylene blue method was established. As compared with H-TdR and MTT methods, methylene blue method possesses features of economic, rapid, convenient and no harmful to the operator, no pollution to the environment. Twenty batches of samples were detected with these three methods. The difference of the results was not significant (p>0.05). Therefore, it is a worthy method to adopt for detecting biological activity of rhIL-2, and suitable for detecting intermediate products during the production of the rhIL-2. |
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| Keywords: | rhIL-2 biological activity detection methylene blue |
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