A method for the quantitative analysis of nerve growth in vitro |
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Authors: | H Meiliu Karl Schmid |
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Institution: | (1) Department of Pathology (Neuropathology), Miriam Hospital and Brown University, 02906 Providence, Rhode Island;(2) Department of Biochemistry, Boston University, School of Medicine, 02118 Boston, Massachusetts |
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Abstract: | Summary A method is described for the quantitative analysis of the nerve-growth-promoting activity of biological molecules in tissue
culture. The criteria used for the evaluation of this activity is based on the neurite length as well as the total number
of neurites produced by the explant of whole dorsal root ganglia from 12-d-old chick embryos. A nerve growth index (NGI) is
given to each ganglion during each of a 5-d culture period. The NGI is defined as the product of average neurite length in
millimeters and the total number of neurites. We report that with increasing concentrations of fetal bovine serum, there was
a proportional increase in NGI due to increased neurite density while the neurite length was not greatly affected. The NGI
of several proteins with known nerve growth promoting activity, namely nerve growth factor, insulin, transferrin, and fibronectin
were investigated for their activity and compared with that of fetal bovine serum.
This work was supported in part by grant GM-10374 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | nerve growth factor ganglion culture fetal bovine serum insulin transferrin fibronectin |
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