Application of multistage continuous fermentation for production of fuel alcohol by very-high-gravity fermentation technology |
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Authors: | Bayrock D P Michael Ingledew W |
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Institution: | (1) Department of Applied Microbiology and Food Science, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada S7N 5A8, CA |
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Abstract: | A fermentation system to test the merging of very-high-gravity (VHG) and multistage continuous culture fermentation (MCCF)
technologies was constructed and evaluated for fuel ethanol production. Simulated mashes ranging from 15% to 32% w/v glucose
were fermented by Saccharomyces cerevisiae and the dilution rates were adjusted for each glucose concentration to provide an effluent containing less than 0.3% w/v
glucose (greater than 99% consumption of glucose). The MCCF can be operated with glucose concentrations up to 32% w/v, which
indicates that the system can successfully operate under VHG conditions. With 32% w/v glucose in the medium reservoir, a maximum
of 16.73% v/v ethanol was produced in the MCCF. The introduction of VHG fermentation into continuous culture technology allows
an improvement in ethanol productivity while producing ethanol continuously. In comparing the viability of yeast by methylene
blue and plate count procedures, the results in this work indicate that the methylene blue procedure may overestimate the
proportion of dead cells in the population. Ethanol productivity (Yps) increased from the first to the last fermentor in the
sequence at all glucose concentrations used. This indicated that ethanol is more effectively produced in later fermentors
in the MCCF, and that the notion of a constant Yps is not a valid assumption for use in mathematical modeling of MCCFs. Journal of Industrial Microbiology & Biotechnology (2001) 27, 87–93.
Received 20 January 2001/ Accepted in revised form 28 April 2001 |
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Keywords: | : very-high-gravity (VHG) multistage continuous culture fuel alcohol Saccharomyces cerevisiae |
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