Sensitivity comparison between Mini-FLOTAC and conventional techniques for the detection of Echinococcus multilocularis eggs |
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| Affiliation: | 1. Laboratory of Parasitology, Faculty of Veterinary Medicine, Hokkaido University, Kita 18, Nishi 8, Sapporo, Hokkaido 060-0818, Japan;2. Department of Infectious Diseases, Hokkaido Institute of Public Health, Kita 19, Nishi 12, Sapporo, Hokkaido 060-0819, Japan;3. Department of Infectious Diseases, Division of Parasitology, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan;1. Ordu University, Fatsa Faculty of Marine Sciences, 52400 Ordu, Turkey;2. Sinop University, Faculty of Fisheries and Aquatic Sciences, 57000 Sinop, Turkey;3. Kocaeli University, Faculty of Agricultural and Natural Sciences, 41285 Kocaeli, Turkey;1. Department of Zoology, University of Sargodha, Sargodha, Pakistan;2. Department of Zoology, The Women University Multan, Multan, Pakistan;3. Department of Entomology, University of Sargodha, Sargodha, Pakistan;4. Department of Zoology, Institute of Ecology and Earth Sciences, University of Tartu, Tartu, Estonia;5. Department of Veterinary Medicine, University of Sassari, Sassari, Italy;1. Laboratory of Animal Health, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen-kibanadai-nishi-1-1, Miyazaki 889-2192, Japan;2. Center for Animal Disease Control (CADIC), University of Miyazaki, Gakuen-kibanadai-nishi-1-1, Miyazaki 889-2192, Japan;3. Laboratory of Veterinary Parasitic Diseases, Department of Veterinary Sciences, Faculty of Agriculture, University of Miyazaki, Gakuen-kibanadai-nishi-1-1, Miyazaki 889-2192, Japan;4. Laboratory of Parasitology, Graduate School of Infectious Diseases, Faculty of Veterinary Medicine, Hokkaido University, Kita 8, Nishi 5, Kita-ku, Sapporo, Hokkaido 060-0818, Japan |
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| Abstract: | Canines serve as the definitive host of Echinococcus multilocularis. This study evaluated the sensitivity of the Mini-FLOTAC technique (MF) for the detection of E. multilocularis eggs in definitive hosts. First, we investigated the effects of heat inactivation and preservative conditions on the detection rate of eggs obtained from experimentally infected dogs. The sensitivity of MF was compared with that of eight other techniques: the centrifugal flotation with sucrose or zinc sulfate, MGL, AMS III, and a combination of MF and flotation/sedimentation techniques. Finally, we compared the sensitivity of MF and the centrifugal flotation with sucrose for the feces of E. multilocularis-infected foxes. The detection rate reached a plateau level with a specific gravity (s.g.) 1.22 for fresh eggs, but the highest rates were obtained with s.g. greater than 1.32 for heat-inactivated eggs. There was no significant difference in the detection rate among the preservative conditions. MF showed significantly higher EPG than the other techniques. Moreover, it showed higher diagnostic sensitivity for the fox feces than the centrifugal flotation technique. These results suggest that heat inactivation may alter s.g. of E. multilocularis eggs and that MF with zinc sulfate (s.g. = 1.32) would be effective for detecting heat-inactivated E. multilocularis eggs. |
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