Antioxidant activity-guided isolation of flavonoids from Silene gallica aerial parts |
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| Institution: | 1. Université des frères Mentouri-Constantine 1, Département de chimie, Laboratoire d′Obtention de Substances Thérapeutiques (LOST), Campus Chaabet-Ersas, 25000 Constantine, Algeria;2. Université de Reims Champagne Ardenne, CNRS, ICMR UMR 7312, 51097 Reims, France;1. College of Pharmacy, Fujian University of Traditional Chinese Medicine, No.1 Qiuyang Road, Fujian 350108, PR China;2. Department of Phytochemistry, School of Pharmacy, Naval Medical University, No. 325, Guohe Road, Shanghai 200433, PR China;3. School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, Yunnan, PR China;1. Facultad de Ciencias Químicas, Universidad Autonoma de Nuevo Leon, Av. Universidad S/N, Ciudad Universitaria, CP 66451 San Nicolás de los Garza, Nuevo León, Mexico;2. Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina/Hospital Universitario, Dr. José Eleuterio González, Universidad Autonoma de Nuevo Leon, Madero y Aguirre Pequeño, Mitras Centro, Monterrey, Nuevo León C.P. 64460, Mexico;3. Departamento de Química Analítica, Facultad de Medicina, Universidad Autonoma de Nuevo Leon. Madero y Aguirre Pequeño, Mitras Centro, Monterrey, Nuevo León C.P. 64460, Mexico;4. Nantes Université, Cibles et médicaments des infections et de l′immunité, IICiMed, UR 1155, F-44000 Nantes, France;1. School of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China;2. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Science, Northwest University, Xi’an 710069, China;1. Department of Cosmeceutics, China Medical University, Taichung 406040, Taiwan;2. Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 40402, Taiwan;3. Chinese Medicine Research Center, China Medical University, Taichung 40402, Taiwan;4. Graduate Institute of Integrated Medicine, China Medical University, Taichung 40402, Taiwan;5. Proteomics Core Laboratory, Department of Medical Research, China Medical University Hospital, Taichung 40402, Taiwan |
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| Abstract: | A bio-guided fractionation of the 80% aqueous ethanolic extract of the aerial parts of Silene gallica L. (Caryophyllaceae), growing in North-Eastern Algeria, was performed to evaluate its antioxidant activity using DPPH, hydroxyl radical scavenging and CUPRAC assays. Successive chromatographic separations of the most antioxidant n-BuOH soluble fraction yielded four acylated flavone C-glycosides, vitexin 2''-O-β-d-(4''',6'''-di-acetyl)-glucopyranoside (1), orientin-2''-O-β-d-(4''',6'''-di-acetyl)-glucopyranoside (2), orientin-2''-O-β-d-(6'''-feruloyl)-glucopyranoside (3), and orientin-2''-O-β-d-(6'''-sinapoyl)-glucopyranoside (4), as well as six known compounds including four flavonoids (5-8), a phenylpropanoid glycerolglucoside (regaloside A) (9), and a phytoecdysteroide (20-hydroxyecdysone) (10). Their structures were established by UV, 1D, 2D NMR, and HR-ESI-MS spectral data, in addition to comparison with literature data. The antioxidant activity of the crude extracts, fractions and compounds 1-8 was evaluated. Two acylated orientin glycosides (3 and 4) displayed the strongest antioxidant activity. |
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| Keywords: | Flavonoid Antioxidant DPPH HORAC CUPRAC |
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