Pampas fox (Lycalopex gymnocercus) of the Argentine Pampas as intermediate host for Neospora caninum |
| |
| Institution: | 1. Instituto de Investigaciones en Producción, Sanidad y Ambiente (IIPROSAM), Facultad de Ciencias Exactas y Naturales, UNMdP, Funes 3350, Mar del Plata, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina;2. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce), INTA-CONICET, Ruta 226 km 73.5, Balcarce, Argentina;3. Department of Pathology, Microbiology & Immunology, School of Veterinary Medicine, University of California, Davis, CA, United States;4. Departamento de Medio Ambiente Facultad Regional Mar Del Plata, Universidad Tecnológica Nacional, CONICET, Av. Dorrego 281, Mar del Plata, Argentina;5. Laboratorio de Inmunoparasitología, Facultad de Ciencias Veterinarias, UNLP, CONICET, Calle 60 y 118, La Plata, Argentina;6. Facultad de Ciencias Agrarias (FCA), Universidad Nacional de Mar del Plata (UNMdP), 7620 Balcarce, Argentina;1. Key Lab of Environment and Health, School of Public Health, Xuzhou Medical University, No.209, Tongshan Road, Xuzhou 221004, Jiangsu, China;2. Yunnan Institute of Parasitic Diseases, No.6 Xiyuan Road, Simao City, Puer 665000, Yunnan, China;3. Jinan Blood Center, No.127 Jingliu Road, Jinan 250001, Shandong, , China;1. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan;2. Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, South Valley University, Qena, Egypt;3. Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan;4. Division of Pathobiological Science Department of Basic Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan |
| |
| Abstract: | The Pampas fox (Lycalopex gymnocercus) is the most abundant wild canid from South America. This wild canid inhabits grasslands, open woodlands, and areas highly modified by extensive ranching and agricultural activities. We aimed to evaluate Neospora caninum infection in tissues from the Pampas fox from Argentina. A total of 41 free-living Pampas foxes were sampled in rural areas located in the Humid Pampas region, Argentina. Brain tissue and different muscles were assessed by histologic and molecular methods. No N. caninum cysts were observed in brain and muscle tissue samples analyzed by histology and immunohistochemistry. Molecular N. caninum identification from brain tissue was based on amplification by PCR of Nc-5 gene and ITS1 rRNA fragments and subsequent sequencing. The presence of N. caninum DNA was 74% (23/31) for the Nc-5 gene and was confirmed by a second ITS1 PCR in 55% (17/31) of the brain tested. Thirteen ITS1 consensus sequences were obtained, and all have a 99.58–100% similarity with N. caninum reference sequences. Only 4% (1/23) of muscles samples analyzed were positive for the Nc-5 gene of N. caninum. This study demonstrated a high prevalence of N. caninum DNA in brain from free-ranging Pampas fox of the Pampa Argentine, thus confirming that this wild canid is a wide distributed intermediate host. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
