Comparative study of oxidative stress and antioxidative markers in patients infected with Toxoplasma gondii |
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| Institution: | 1. Department of Ophthalmology, Federal University of Sao Paulo, Sao Paulo, Brazil;2. Universidade Regional Integrada Alto Uruguai e Missões, Sete de Setembro Avenue, 1621, Erechim, Rio Grande do Sul 99709-910, Brazil;3. Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, BR 472, Km 585, Uruguaiana, RS, Brazil;4. Clinica Silveira, Sete de Setembro Avenue, 1502, Erechim, Rio Grande do Sul, Brazil;1. Department of Dermatology, Asahi General Hospital, Chiba 289-2511, Japan;2. Institute for Research Management, Oita University, Oita 879-5593, Japan;3. Department of Diagnostic Pathology, Asahi General Hospital, Chiba 289-2511, Japan;4. Department of Health, Sports, and Nutrition, Faculty of Health and Welfare Studies, Kobe Women''s University, Kobe 650-0046, Japan;5. National Collection of Animal Helminths, Epidemiology, Parasites and Vectors Programme, ARC-Onderstepoort Veterinary Institute, Private Bag X05 Onderstepoort 0110, South Africa;6. Department of Environmental Medicine, Kochi Medical School, Kochi University, Nankoku 783-8505, Japan;7. Department of Biomedicine, Faculty of Medicine, Oita University, Oita 879-5593, Japan;8. Department of Infectious Diseases, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan;9. Agriculture Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8666, Japan;10. Department of Parasitology, Kurume University School of Medicine, Kurume 830-0011, Japan;11. UMR7245 MCAM, Muséum National d''Histoire Naturelle, CNRS, CP52, 61 rue Buffon, 75005 Paris, France;12. Tropical Infectious Diseases Research and Education Centre, Universiti Malaya, 50603 Kuala Lumpur, Malaysia;1. Laboratory of Parasitology, Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain;2. Sorex Ecoloxía e Medio Ambiente S.L.U., Rúa das Barreiras 80,15702 Santiago de Compostela, A Coruña, Spain;3. Research Institute on Chemical and Biological Analysis, University of Santiago de Compostela, Campus Vida, 15782 Santiago de Compostela, A Coruña, Spain;1. School of Science, Mae Fah Luang University, Chiang Rai, Thailand;2. Gut Microbiome Research Group, Mae Fah Luang University, Chiang Rai, Thailand;3. Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury, United Kingdom;1. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry and University Hospital, Palacky University Olomouc, Czech Republic;2. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Solna, Sweden;3. A.V. Bogatsky Physico-Chemical Institute of National Academy of Sciences of Ukraine, Odessa, Ukraine;4. I.I. Mechnikova Odessa National University, Odessa, Ukraine |
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| Abstract: | Recently, oxidative stress and antioxidative compounds have been described as potential biomarkers. However, there is no consensus on the most appropriate oxidative and antioxidative biomarkers for patients with Toxoplasma gondii. In the present study, we evaluated the levels of lipid, protein, DNA oxidative damage and antioxidants in samples from patients infected with T. gondii with and without ocular toxoplasmosis. The levels of MDA, TBARS, micronuclei, carbonyl, GSH, vitamin C and vitamin E were measured on samples from 8 patients positive for T. gondii antibodies with ocular toxoplasmosis (OT), 20 patients positive for T. gondii antibodies without ocular toxoplasmosis (non OT), and 12 healthy individuals negative for T. gondii antibodies. The levels of MDA, TBARS, carbonyl and micronuclei were significantly higher in non OT patients, while MDA and TBARS levels were lower in OT patients. In contrast, the antioxidative factors, GSH and vitamin E levels were significantly lower in non OT patients, while vitamin C was lower in non OT and OT patients. Additionally, non OT patients were indicated to be high producers of oxidative markers (TBARS, MDA, micronuclei and carbonyl), while control group was indicated to be high producer of antioxidative markers (GSH, vitamins C and E). However, OT patients were not found as high producers of oxidative nor antioxidative markers. Our results provide a starting point of possible markers to better understand the disease pathogenesis in patients infected with T. gondii. Additional studies are needed to clarify the potential contribution of oxidative and antioxidative markers in these patients population. |
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