Molecular analyses confirm the coexistence of Fasciola gigantica and parthenogenetic Fasciola in the Philippines |
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| Affiliation: | 1. Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-8550, Japan;2. Department of Parasitology, College of Public Health, University of the Philippines, Manila 1000, Philippines;1. Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonosis, Yangzhou, Jiangsu Province 225009, PR China;2. Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, PR China;1. Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020–8550, Japan;2. Center for Biotechnology, Agriculture and Forestry University, Rampur, Chitwan, Nepal;3. Institute of Agriculture and Animal Science, Tribhuvan University, Kirtipur, Chitwan, Nepal;4. Regional Veterinary Diagnostic Laboratory, Pokhara, Nepal;5. Himalayan College of Agricultural Sciences and Technology, Kathmandu, Nepal;1. Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, Ueda, 3-18-8, Morioka 020-8550, Japan;2. Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University, Yanagido 1-1, Gifu 501-1193, Japan;3. Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim Manipal University, Gangtok, Sikkim, India;4. Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan;1. Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan;2. Department of Pathogenetic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;3. National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Nishi, Inada-cho, Obihiro, Hokkaido 080-8555, Japan;4. Laboratory of Veterinary Microbiology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan;5. Department of Parasitology, Asahikawa Medical University, Asahikawa, Hokkaido 078-8510, Japan |
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| Abstract: | Fasciola flukes collected from domestic buffalos and cattle in the Philippines were confirmed as Fasciola gigantica and parthenogenetic Fasciola based on DNA analyses of nuclear pepck and pold genes, and the mitochondrial ND1 gene. This study is the first to elucidate that F. gigantica and parthenogenetic Fasciola coexist in the Philippines with prevalences of 90.6% and 9.4%, respectively. The F. gigantica population showed a high genetic diversity with 25 ND1 haplotypes, suggesting that F. gigantica has existed in the Philippines for a long time. In contrast, parthenogenetic Fasciola flukes showed a single ND1 haplotype (Fsp-ND1-P1), which was identical to the founder haplotype, Fg-C2 of parthenogenetic Fasciola in China. These results indicate that parthenogenetic Fasciola in the Philippines is a recently introduced population from a neighboring continent. |
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