Thermal proteome profiling: Insights into protein modifications,associations, and functions |
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| Affiliation: | 1. Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research–UFZ, Permoserstraße 15, 04318 Leipzig, Germany;2. Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University, 07743 Jena, Germany;3. Department of Isotope Biogeochemistry, Helmholtz Centre for Environmental Research–UFZ, Permoserstraße 15, 04318 Leipzig, Germany;4. Chair of Geobiotechnology, Technische Universität Berlin, Ackerstraße 76, 13355 Berlin, Germany;5. Institute of Biochemistry, Faculty of Life Sciences, University of Leipzig, Brüderstraße 34, Germany;1. Department of Food Science and Biotechnology, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan, ROC;2. Institute of Biomedical Science, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan, ROC;1. State Key Laboratory of Fine Chemicals, School of Life Science and Technology, Dalian University of Technology, Dalian, China;2. State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian, China;3. Department of Hematology, Second Affiliated Hospital, Dalian Medical University, Dalian, China |
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| Abstract: | Tracking proteins’ biophysical characteristics on a proteome-wide scale can provide valuable information on their functions and interactions. Thermal proteome profiling (TPP) is a multiplexed quantitative proteomics approach that measures changes in protein thermal stability—a key biophysical property—across different cellular states. Developed in 2014, as a target-deconvolution assay for drugs and other small molecules, TPP has since evolved to a system-level biochemical omics technique providing insights into context-dependent changes in protein states. In this review, we summarise key advances in the experimental and data analysis pipeline that have aided this transformation and discuss the recent developments and applications of TPP. |
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| Keywords: | Thermal proteome profiling TPP MS-CETSA Proteomics Post-translational modifications PTMs Protein complexes Bottom-up proteomics |
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