Emerging fluorescence tools for the study of proteostasis in cells |
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| Institution: | 1. Department of Biochemistry and Chemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia;2. ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Victoria, 3010, Australia;1. Center for Systems Biology, Massachusetts General Hospital Research Institute, 185 Cambridge St, Boston, MA 02114, United States;2. Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 55 Fruit St, Boston, MA 02114, United States;3. Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Harvard Medical School, 450 Brookline Ave, Boston, MA 02115, United States;4. Department of Radiology, Brigham and Women''s Hospital, Harvard Medical School, Boston, MA 02215, United States;1. Institute of Biochemistry, University of Münster, Germany;2. European Institute for Molecular Imaging (EIMI), University of Münster, Germany;3. Department of Nuclear Medicine, University Hospital Münster, Germany;1. Laboratory of Cancer Biology, Department of Molecular Biology, Ariel University, Ariel, 40700, Israel |
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| Abstract: | Understanding how cells maintain the functional proteome and respond to stress conditions is critical for deciphering molecular pathogenesis and developing treatments for conditions such as neurodegenerative diseases. Efforts towards finer quantification of cellular proteostasis machinery efficiency, phase transitions and local environment changes remain a priority. Herein, we describe recent developments in fluorescence-based strategy and methodology, building on the experimental toolkit, for the study of proteostasis (protein homeostasis) in cells. We hope this review can assist in bridging gaps between a multitude of research disciplines and promote interdisciplinary collaboration to address the crucial topic of proteostasis. |
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| Keywords: | Fluorescent probes Proteostasis Protein misfolding Protein aggregation Fluorescence imaging |
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