The bright frontiers of microbial metabolic optogenetics |
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| Affiliation: | 1. Department of Molecular Biology, USA;2. Department of Chemical and Biological Engineering, USA;3. The Andlinger Center for Energy and the Environment, USA;4. High Meadows Environmental Institute, Princeton University, Princeton NJ 08544, USA;1. Department of Chemistry, University of California, Berkeley, CA, 94720, USA;2. Department of Molecular & Cell Biology, University of California, Berkeley, CA, 94720, USA;3. Helen Wills Neuroscience Institute, University of California, Berkeley, CA, 94720, USA;1. Department of Bioinformatic Engineering, Graduate School of Information Science and Technology, Osaka University, Yamadaoka, Suita, Osaka, 565-0871, Japan;2. Department of Environmental and Life Sciences, School of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan;3. Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo, 657-8501, Japan;4. Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Nagoya, Aichi, 466-8555, Japan;5. OptoBioTechnology Research Center, Nagoya Institute of Technology, Showa-ku, Nagoya, Aichi, 466-8555, Japan;6. Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan;7. Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe, Hyogo, 657-8501, Japan;1. Division of Plant Environmental Responses, National Institute for Basic Biology, National Institutes of Natural Sciences, 38 Nishigonaka, Myodaiji, Okazaki, Japan;2. PRESTO, Japan Science and Technology Agency, 4-1-8, Honcho, Kawaguchi, Japan;3. Technical University of Munich, School of Life Sciences, Plant Systems Biology, Emil-Ramann-Strasse 8, 85354 Freising, Germany;1. Center for Translational Cancer Research, Institute of Biosciences and Technology Texas A&M University, Houston, TX 77030, USA;2. Center for Epigenetics and Disease Prevention, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA;3. Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M University, Bryan, TX 77807, USA;4. Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China;5. Department of Medical Physiology, College of Medicine Texas A&M University, Temple, TX 76504, USA, USA |
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| Abstract: | In recent years, light-responsive systems from the field of optogenetics have been applied to several areas of metabolic engineering with remarkable success. By taking advantage of light's high tunability, reversibility, and orthogonality to host endogenous processes, optogenetic systems have enabled unprecedented dynamical controls of microbial fermentations for chemical production, metabolic flux analysis, and population compositions in co-cultures. In this article, we share our opinions on the current state of this new field of metabolic optogenetics.We make the case that it will continue to impact metabolic engineering in increasingly new directions, with the potential to challenge existing paradigms for metabolic pathway and strain optimization as well as bioreactor operation. |
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| Keywords: | Metabolic engineering Optogenetics Cybergenetics Biosensors Control Dynamic control of microbial fermentations: Design build test learn cycle DBTL Multivariate modular metabolic engineering MMME |
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