Molecular link between the metacercariae and adults of four species Haematoloechus (Digenea: Plagiorchioidea), including scanning electron microscopy characterization |
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| Institution: | 1. Posgrado en Ciencias Biológicas, Instituto de Biología, Universidad Nacional Autónoma de México, s/n, Ciudad Universitaria, Coyoacán, Ciudad de México C.P. 04510, Mexico;2. Instituto de Biología, Universidad Nacional Autónoma de México, Tercer circuito exterior s/n, Ciudad Universitaria, Coyoacán, Ciudad de México C.P. 04510, Mexico;1. Departamento de Parasitologia Animal, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ, Brazil;2. Fundação Instituto Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios, Av. Brasil 44365, Rio de Janeiro, RJ, Brazil;3. Laboratório de Helmintologia Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade do Estado do Rio de Janeiro, Av. Professor Manoel de Abreu, 444, Vila Isabel, RJ 20550-170, Brazil;1. INRAE, UMR 1282 Infectiologie et Santé Publique, F-37380 Nouzilly, France;2. Université de Tours, UMR 1282 Infectiologie et Santé Publique, F-37000 Tours, France;3. Scotland''s Rural College (SRUC), Animal and Veterinary Sciences, Roslin Institute Building, Midlothian EH25 9RG, UK;4. Facultad de Ciencias Veterinarias, Producción animal, Universidad Nacional de La Pampa, Calle 5 esq. 116 S/N, General Pico, Argentina;1. Instituto de Ecología Aplicada, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas 87019, Mexico;2. Laboratorio de Helmintología, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;3. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Tamaulipas, Ciudad Victoria, Tamaulipas 87000, Mexico;4. Unidad Mérida del Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Mérida, Yucatán 97310, Mexico;1. Laboratory of Veterinary Parasitology, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, Japan;2. Suma Aqualife Park KOBE, 1-3-5 Wakamiyacho, Suma, Kobe, Hyogo, Japan;3. Kyoto Institute of Nutrition & Pathology Inc, 7-2 Furuiketani, Tachikawa, Ujitawaracho, Tsuzuki, Kyoto, Japan;1. Aquatic Parasitology Laboratory, Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture, Auburn University, 559 Devall Drive, Auburn, AL 36832, USA;2. Biology Department and Biological Field Station, 120 Perna Science Building, State University of New York, College at Oneonta, Oneonta, New York 13820, USA;3. North Carolina Wildlife Resources Commission, 645 Fish Hatchery Road, Marion, NC 28752, USA;4. Department of Zoology, School for Environmental Sciences and Development, North-West University, Private Bag X6001, Potchefstroom 2520, South Africa |
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| Abstract: | Haematoloechus comprises a species-rich genus of trematodes commonly found in the lungs of amphibians across the globe. Of the approximately 70 species reported worldwide, Mexico stands out as a biodiversity hot spot for the genus, with 16 species described. In Los Tuxtlas tropical rainforest, in south-eastern Mexico, three species have been reported, two of them endemic of the frog species occurring in the area. Here, we added the record of a fourth species in the area, and partially elucidated the life cycle of these species using DNA sequence data from the 28S rRNA and the COI genes. Frogs, and a diverse array of species of aquatic insects were sampled in two lakes within the tropical rain forest. Adults and metacercariae of Haematoloechus were obtained. Both developmental stages were linked molecularly contributing to our understanding of their transmission pathways, and probable host-specificity patterns. Each of the four species of Haematoloechus were sampled in a particular species of anisopteran odonate, and therefore were considered as specialists towards their second intermediate host. We validated the presence of H. complexus in the region of Los Tuxtlas because their metacercariae, sampled in the anisopteran dragongly Agriogomphus tumens, matched with sequences of H. complexus from Nebraska and Illinois in USA. We further described the ultrastructure of the tegument of three of the four species of Haematoloechus, sampled from their definitive host (Rana vaillanti, Rana berlandieri), to contribute with characters that can be reliable for species delimitation of the genus. |
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