Detection and characterization of Kudoa thunni from uncooked yellowfin tuna (Thunnus albacares) in Southeast Asia |
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| Institution: | 1. Faculty of Fisheries, Vietnam National University of Agriculture, Hanoi, Viet Nam;2. Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi, Viet Nam;3. Fish Health Platform, Centex of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok, Thailand;4. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand;5. Department of Food, Agriculture and Bioresources, School of Environment, Resources & Development (SERD), Asian Institute of Technology (AIT), Klong Luang, Pathumthani, Thailand;1. Department of Microbiology, Oregon State University, Corvallis, OR, USA;2. US Geological Survey, Western Fisheries Research Center, Seattle, WA, USA;1. Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture, Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA;2. North Carolina Wildlife Resources Commission, 645 Fish Hatchery Road, Marion, NC 28752, USA;1. College of Life Science, Hubei Key Laboratory of Edible Wild Plants Conservation & Utilization, Huangshi Biomedicine Industry and Technology Research Institute Company Limited, Hubei Normal University, Huangshi, Hubei 435002, China;2. International Medical School, Hebei Foreign Studies University, Shijiazhuang, Hebei 050096, China;3. United States Department of Agriculture, Agriculture Research Service (USDA-ARS), Animal Genomics and Improvement Laboratory, Beltsville, MD 20705, USA;4. CSIRO Agriculture and Food, Armidale, NSW, Australia;1. Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Rua dos Bragas No. 289, 4050-123 Porto, Portugal;2. Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Rua Jorge Viterbo Ferreira No. 228, 4050-313 Porto, Portugal;3. Department of Sciences, High Institute of Health Sciences — North, CESPU, Rua Central da Gandra No. 1317, 4585-116 Gandra, Portugal;4. Department of Biology, Faculty of Sciences (FCUP), University of Porto, Rua do Campo Alegre, s/n, FC4, 4169-007 Porto, Portugal;5. Zoology Department, College of Sciences, King Saud University, Riyadh 11451, Saudi Arabia |
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| Abstract: | Myxosporean parasites Kudoa spp. have been reported in several marine fish species worldwide. However, little is known about the contamination of these parasites in raw fish in Southeast Asia, where the consumption demand of uncooked fish is increasing. In 2019, the occurrence of several cases of raw yellowfin tuna (Thunnus albacares) obtained from retail shops with the presence of unknown white, nodular cysts within the musculature have raised public health concerns for the consumption of raw marine fish in Vietnam. Microscopic examination revealed numerous myxospores with the quadratic shape of the Kudoidae. Morphologically, stained spores detected in this study are suspected to Kudoa thunni. To confirm the suspected Kudoa species, further examination of the 18S small-subunit (SSU) was conducted and the results of nucleotide sequence analysis obtained from nodular cysts revealed 99.18–100% identity to that of Kudoa thunni sequences available in GenBank. Detection of K. thunni infection in tuna in Southeast Asia highlights the need for appropriate surveillance and control measures to ensure high quality standards and safety on raw fish production and consumption. |
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