酶法脱蛋白提取大枣多糖工艺的研究

讨论了从提取环磷酸腺苷后的枣汁中提取枣多糖的最佳工艺条件,包括枣汁浓缩4倍,加无水乙醇调枣汁中乙醇体积分数为60%,醇沉5 h;木瓜蛋白酶脱蛋白效果最佳,木瓜蛋白酶液与枣汁的体积比为0.4∶ 1,温度60℃、pH5.0,酶解90 min,蛋白脱除率可达91.8%;AB-8树脂脱色,脱色率为73.64%,多糖得率为94.4%.红外光谱显示,提取的多糖与常规方法提取的多糖成分相同.

Research on the Technology Process of Polysaccharide from Zizyphus jujuba MILL by Enzymolysis

This paper studied technology of extracting polysaccharide from jujube juice which has been extracted c-AMP. The extracting liquid contract 4 times, adding ethanol to make the concentration of alcohol 60%, and time was 5 h. Papain was used to remove protein, and the optimum conditions including 0.4∶ 1V/V papain, temperature 60℃, pH5.0, 90 min. This method may increase the removal rate of protein to 91.8%, and the loss rate of polysaccharides in reduced greatly. The extracted solution was then decolorized through macro-reticular adsorption resin column. The rate was 73.64% and the yield of polysaccharide was 94.4%. The structure was similar to the polysaccharide extracted by normal technology.