Immunolocalization of albumin and transferrin in germ cells and Sertoli cells during rat gonadal morphogenesis and postnatal development of the testis |
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Authors: | Jean-Louis Gelly Jean-Pierre Richoux Georges Grignon |
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Affiliation: | (1) Laboratoire d'Histologie-Embryologie, Faculté de Médecine, BP 184, F-54505 Vandoeuvre-les-Nancy Cedex, France |
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Abstract: | The localization of albumin and transferrin was examined immunohistochemically in germ cells and Sertoli cells during rat gonadal morphogenesis and postnatal development of the testis. These proteins appeared as early as the 13th day of gestation in migrating primordial germ cells before Sertoli cell differentiation. In the fetal testis, strong immunoreactivity was only detected in the gonocytes. In the prepubertal testis, spermatogonia, primary spermatocytes, and some Sertoli cells accumulate albumin and transferrin. At puberty, different patterns of immunostaining of the germ cells were observed at the various stages of the cycle of the seminiferous epithelium. Diplotene spermatocytes at stage XIII, spermatocytes in division at stage XIV, and round spermatids at stages IV–VIII showed maximal staining. Labeling was evident in the cytoplasm of adult Sertoli cells. Albumin and transferrin staining patterns paralleled each other during ontogenesis. |
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Keywords: | Albumin Transferrin Germ cells Sertoli cells Testis Immunocytochemistry Ontogenesis Rat (Wistar) |
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