蒜鳞片休眠进程中胞间联络的变化及类外连丝结构与功能的研究

利用电子显微镜术,对蒜休眠进程中鳞片薄壁细胞间胞间联络的特征进行了实验观察,发现不同时期胞间联络具有随细胞间生理关系密切程度而呈现相应结构变化的特点。并观察到萌芽期鳞片中衰败细胞与存活细胞之间有类外连丝型胞间连丝的存在;以激光共聚焦荧光显微镜结合荧光标记物示踪检测,发现不透膜荧光物质分子量为457Da 的萤黄(Lucifer yellow,LYCH),可以共质体运输方式进入存活细胞内,论证了类外连丝这一胞间连丝特定修饰态的存在,并可在一段时间内继续保持生理活性,起到进行物质共质运输的功能。

STUDIES ON THE CHANGE OF INTERCELLULAR CONNECTION AND THE APPEARANCE AND FUNCTION OF ECTODESMATA-LIKE STRUCTURE IN GARLIC CLOVE DURING THE DORMANCY DEVELOPMENT

The characteristics of intercellular connection have been observed during the dor-mancy development of garlic by the transmission electron microscope (TEM).The results indicatedthat the appearances of the different states of intercellular connection were physiological adaptationto the development of garlic clove during storage.Ectodesmata-like existed at the wall boundary be-tween the declining tissue and the living cells in the garlic during germination period.By ConfocalLaser Scanning Microscopy (CLSM) combined with fluorescence probe.457Da Lucifer Yellow(LYCH) which was impermeable to the membrane,could enter the living parenchyma cell throughsymplastic route.The result proved that ectodesmata-like structure,which may be recognized asthe modified plasmodesata,still retained physiological activity during a certain time and functionas the channels of the symplastic transport of nutrient.